Journal: Cell Death & Disease

Article Title: Androgen receptor-dependent DRAM1 activation drives autophagic resistance to BRAF inhibitors in BRAFV600-mutant melanoma

doi: 10.1038/s41419-026-08547-x

Figure Lengend Snippet: A Bioinformatics prediction of putative AR binding sites (androgen response elements, AREs) in the 5’-promoter region of DRAM1 using the JASPAR database. Dual luciferase assays assessing DRAM1 promoter activity in ( B , C ) A375R and WM793R cells transfected with si-AR or D , E A375 and WM793 cells transfected with AR overexpression. ChIP assays using anti-AR antibody in ( F , G ) AR OE A375 or WM793 cells or H , I AR knockdown A375R and WM793R cells. J Schematic of mutated ARE sequences in the DRAM1 5’-promoter region. Dual luciferase assays in ( K , L ) AR OE A375 and WM793 cells or M , N AR knockdown A375R and WM793R cells transfected with wildtype (WT) or mutant (MT) DRAM1 promoter constructs. Mean ± SEM, n = 3, two-tailed Student’s t test. * p < 0.05; ** p < 0.01; *** p < 0.001; ns no significance.

Article Snippet: Lysates were immunoprecipitated overnight at 4 °C with 5 μg anti-AR (CST, #5153) or IgG control (Proteintech, #30000-0-AP), followed by 3 h incubation with Protein G Magnetic Beads (CST, #9006).

Techniques: Binding Assay, Luciferase, Activity Assay, Transfection, Over Expression, Knockdown, Mutagenesis, Construct, Two Tailed Test